Dr. NITI PURI
Assistant Professor
School of Life Sciences
Jawaharlal Nehru University
New Delhi-110067
 

 

Primary Research Focus     

Molecular mechanisms of regulated Exocytosis from mast cells.    Intracellular protein traffic in Immune cells.

Career                       

  • May, 2008 onwards: Assistant Professor in Immunology, in the School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

  • October, 2005 till April, 2008 : Visiting Fellow (Employee) in the Laboratory of Dr. Paul A. Roche, Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

  • April, 2005 till September, 2005 : Visiting Fellow (Contract Employee) in the Laboratory of Dr. Paul A. Roche, Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

  • March, 2000 till March, 2005 : Post Doctoral Visiting Fellow in the Laboratory of Dr. Paul A. Roche, Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

  • September, 1997 till Nov 1999 : Project Associate in the project titled, " Cloning, Expression and Purification of Ovine Growth Hormone" funded by Dept. of Biotechnology, Govt. of India, with Dr. Satish M. Totey, Embryo Biotechnology Lab., National Institute of Immunology, New Delhi, as the Principal Investigator.

  • December, 1995 till December, 1996: Research Associate in the Project titled, "Major Histocompatibility Complex I Antigen  Expression Augmenting Factor (MEAF), A new immunomodulatory Cytokine : Purification, characterization and sequencing " funded by Dept. of Biotechnology, Govt. of India, with Prof. Rajiv K. Saxena, Jawaharlal Nehru University, New Delhi, as the Principal Investigator.

Merits and Fellowships

  • November 1994 till October 1995 :Senior Research Fellow (ICMR) in School of Life   Sciences, J.N.U., New Delhi.  Title of project : "Modulation of certain membrane receptors crucial for immunological interactions, by mouse natural killer-resistance inducing factor (NK-RIF)"

  • August 1991 till July 1994  :Senior Research Fellow (JNU-UGC)in School of Life Sciences, J.N.U., New Delhi

  • August 1989 to July 1991  :Junior Research Fellow (JNU-UGC) in School of Life Sciences, J.N.U., New Delhi

  • Passed with position in top 5% in CSIR-UGC-JRF Exam, 1988

  • Students Fellowship sponsored by Department of Biotechnology, Govt. of India, during M. Sc. (1987-1989)

  • Award for best poster presentation, 18th Annual meeting of the Indian Immunology Society, NII, New Delhi (October 1991)

  • Attended  workshop on flowcytometry at Cancer  Research  Institute, Tata Memorial Centre, Bombay (June 1994).

  • Member of professional organizations: 
                     1.  Indian Immunology Society as a Life Member.
                     2. American Society for Cell Biology.

List of Publications

  1. Raval, A., Puri, N., and Saxena, R. K. (1997) Generation of human class I major histocompatibility complex activating factor in serum free medium and its partial characterization. J. Biosci. 22(1):59-68.

  2. Puri, N., Raval, A., and Saxena, R. K. (1997) Species specificity of a novel factor which augments the expression of MHC class I antigens on tumor cell lines. Exp. Mol. Med. 29(2):129-132.

  3. Raval, A., Puri, N., Rath, P. C., and Saxena R. K. (1998) Cytokine regulation of expression of class I MHC antigens. Exp. Mol. Med. 30(1):1-13.

  4. Puri, N., and Saxena, R. K. (1998) Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells. Exp. Mol. Med. 30(2):93-99.

  5. Puri, N., and Saxena, R. K. (1998) Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells. Exp. Mol. Med. 30(4):199-204.

  6. Puri, N., Appa Rao, K. B. C., Menon, S., Panda, A. K., Tiwari, G., Garg, L. C., and Totey, S. M. (1999) Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli. Protein Expression Purif. 17(2):215-223.

  7. Vaidyanathan, V. V., Puri, N., and Roche, P.A. The last exon of SNAP-23 regulates granule exocytosis      from mast cells.  J. Biol. Chem. 276: 25101-25106, 2001.

  8. Puri, N., Kruhlak, M. J., Whiteheart, S. W. and Roche, P. A. Mast cell degranulation requires N-Ethylmaleimide-Sensitive Factor mediated SNARE disassembly. J. Immunol. 171: 5345-5352, 2003.

  9. Hepp, R., Puri, N. (equal contribution with 1st author), Hohenstein, A. C., Crawford, G. L., Whiteheart, S. W. and Roche, P. A. Phosphorylation of SNAP-23 regulates exocytosis from mast cells. J. Biol. Chem. 280(8): 6610-6620, 2005.

  10. Puri, N. and Roche, P. A. Ternary SNARE complexes are enriched in lipid rafts during mast cell exocytosis. Traffic 7: 1482-1494, 2006.

  11. Puri, N. and Roche, P. A. Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. Proc. Natl. Acad. Sci., U.S.A. 105(7) : 2580-2585, 2008.

Papers presented in conferences and meetings

  1. Puri, N., Sarin, A., and Saxena, R.K. (1991) Partial purification and characterisation of Natural Killer-Resistance Inducing Factor from the culture supernatants of murine mixed lymphocyte cultures. 18th Annual meeting of Indian Immunology Society, NII New Delhi. Oct., 1991.

  2. Puri, N., and Saxena, R.K. (1993) A non-IL-2 proliferation inducing activity in mouse MLC-RIF preparation. XX- Annual Conference of Indian Immunology Society, Bhopal, Dec., 1993.

  3. Puri, N., and Saxena, R. K. (1995) A novel B-cell growth factor released by mouse spleen cells undergoing mixed lymphocyte reaction. XXII Annual Conference of Indian Immunology Society and National Symposium on Immunology of infectious diseases, Jawaharlal Nehru University, New Delhi, Dec., 1995.

  4. Totey, S. M., Babu, S., Puri, N., Mandal, A., Gulati, N., Anand, R., Garg, L., Kumar, V., and Brahmachari, V. (1998) Transgenic mouse models of human diseases. 67th Annual meeting of Society of Biological Chemists, India at Jawaharlal Nehru University, New Delhi, Dec., 1998.

  5. Puri, N., Mishra, M., Panda, A. K., and Totey, S. M. (1999) Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli 68th Annual meeting of Society of Biological Chemists, India at Indian Institute of Science, Bangalore. Dec.,1999.

  6. Puri, N., Gulati, N., Mandal, A., Anand, R., and Totey, S. M. (1999) Transgenic weanling mice expressing Neomycin resistance gene show signs of immunodeficiecy. 68th Annual meeting of Society of Biological Chemists, India at Indian Institute of Science, Bangalore. Dec.,1999. 

  7. Puri, N., and Roche, P. A. (2001) NSF-mediated SNARE “Priming” is required for regulated exocytosis from RBL mast cells. NIH Immunology Retreat, Warrenton, VA, USA, 2001.

  8. Puri, N., and Roche, P. A. (2001) NSF-mediated SNARE “Priming” is required for regulated exocytosis from RBL mast cells. 41st American Society for Cell Biology Annual Meeting, Washington D. C., USA, December 8-12, 2001.

  9. Puri, N., and Roche P. A. (2002) Lipid Raft association of SNARE proteins in RBL mast cells. NIH Immunology Retreat, Warrenton, VA, USA, 2002.

  10. Puri, N., and Roche, P. A. (2002) Lipid raft association of SNAP-23 and other SNARE proteins: implications for regulated exocytosis from RBL mast cells. 42nd American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, December 14-18, 2002.

  11. Puri, N., and Roche, P. A. (2003) Lipid raft association of SNAP-23 and other SNARE proteins: implications for regulated exocytosis from RBL mast cells. NIH Membrane Microdomain Interest Group meeting, Bethesda, MD, USA, April 1st, 2003.

  12. Puri, N., and Roche, P. A. (2004) Ternary SNARE complexes are associated with lipid rafts in RBL mast cells. 44th American Society for Cell Biology Annual Meeting, Washington D. C., USA, December 4-8, 2004.

  13. Puri, N., Hepp, R. and Roche, P. A. (2005) Phosphorylation of SNAP-23 regulates exocytosis from mast cells. NIH Protein Trafficking Interest Group meeting, Bethesda, MD, USA, February, 2005.

  14. Puri, N., and Roche, P. A. (2005) Ternary SNARE complexes are associated with lipid rafts in RBL mast cells. Annual meeting of The American Association of Immunology, San Diego, CA, USA, April 2-6, 2005.

  15. Puri, N., Hohenstein, A. C., and Roche, P. A. (2005) VAMP-8 plays an important role in regulated exocytosis from mast cells but not from CTL. NIH Immunology Retreat, Warrenton, VA, USA, 2005.

  16. Puri, N., and Roche, P. A. (2007) Molecular mechanisms of regulated granule exocytosis from mast cells and CTL. Invited Seminar. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, March 2007.

  17. Puri, N., and Roche, P. A. (2007) Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. NIH Immunology Retreat, Warrenton, VA, USA, October 2007.

  18. Puri, N., and Roche, P. A. (2007) Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. 47th American Society for Cell Biology Annual Meeting, Washington D. C., USA, December 1-5, 2007.