PROF. PRAMOD KUMAR YADAVA

Professor
School of Life Sciences
Jawaharlal Nehru University
New Delhi -110067, India.
Room No.      :
Off. Phone    : 26704520
Residence     : 26184419
Fax                :  011-6165886,
E-mail           : pky@mail.jnu.ac.in

Education

Ph.D. (1976)         Banaras Hindu University.            Genetic Studies on Nitrogen Fixation by Cyanobacteria.

M.Sc.(1972)         Banaras Hindu University.            Botany (Microbial Genetics).

B.Sc. (1970)         Banaras Hindu University             Chemistry, Botany and Zoology.

Career Teaching:

1974-79                 Experimental Phycology to M.Sc. (Botany) at Banaras Hindu University.

1990.-to date         Microbiology, Molecular Biology, Molecular Genetics and Applied Molecular Biology

                            for M.Sc. and M.Phil students at the School of Life Sciences, Jawaharlal Nehru University.

Research:

1977-79 Harvesting of Pollutant-grown Algae and their Use as Animal Feed etc, BHU.
1979-81 Primary Processes in Photosynthesis, JNU.
1981(Jul-Dec) Effects of Pesticides on Soil Microorganisms, JNU.
Dec 1981-Mar1985 Cognitive Mechanisms in Interaction of Nitrogen-fixing Microorganisms with Plants, IISc, Bangalore.
Apr 1985-Oct 1985 Polymorphism in Plant Lectin Genes, IISc, Bangalore.
Oct 1985-Sept 1986 Assessment of Environmental Impact of Certain Industries, as Consultant Scientist to Central India Consultancy Organization, Bhopal.
Oct 1986-Jan 1990 Development of RNA Replicons based on Qb RNA Replicase, as a post-Doctoral Scientist at the Institute of Molecular Biology I, University of Zurich, SWITZERLAND.
1990-current Design and Developement of targeted ribozymes and chimeric flagellin molecules as potent molecular medicines and vaccine candidates, and Construction and rescue of recombinant measles viruses with heterologous inserts JNU, New Delhi.
Jan-Jun 30, 1997 Measles virus reverse genetic system as Visiting Professor at the Institute of Molecular Biology I, University of Zurich, SWITZERLAND, continued at JNU Rescue of Recombinant Measles Virus with heterologous peptides’.
Sept 2003-todate Selection of RNA aptamers binding with chosen ligands, JNU.

Area of Research

Development of Expression and Delivery System for Targeted Ribozymes and Chimeric Peptide Antigens.

Research Projects

  1. 1972-76: Genetic Studies on Nitrogen Fixation by Blue-green Algae, BHU.

  2. 1977-79: Harvesting of Pollutant-grown Algae and their Use as Animal Feed etc, BHU.

  3. 1979-81: Primary Processes in Photosynthesis, JNU.

  4. 1981(Jul-Dec): Effects of Pesticides on Soil Microorganisms, JNU.

  5. Dec 1981-Mar1985: Cognitive Mechanisms in Interaction of Nitrogen-fixing Microorganisms with Plants, IISc, Bangalore.

  6. Apr 1985-Oct 1985: Polymorphism in Plant Lectin Genes, IISc, Bangalore.

  7. Oct 1985-Sept 1986: Assessment of Environmental Impact of Certain Industries, as Consultant Scientist to Central India Consultancy Organization, Bhopal.

  8. Oct 1986-Jan 1990: Development of RNA Replicons based on Qb RNA Replicase, as a post-Doctoral Scientist at the Institute of Molecular Biology I, University of Zurich, SWITZERLAND.

  9. 1990-current: Design and Developement of targeted ribozymes and chimeric flagellin molecules as potent molecular medicines and vaccine candidates, and Construction and rescue of recombinant measles viruses with heterologous inserts JNU, New Delhi.

  10. Jan-Jun 30, 1997, Measles virus reverse genetics system as Visiting Professor at the Institute of Molecular Biology I, University of Zurich, SWITZERLAND, continued at JNU ‘Rescue of Recombinant Measles Virus with hetrologous peptides’.Sept.2003, Selection of RNA apartmers binding with chosen ligands, JNU.

  11. Construction of targeted ribozyme against RNA component of telomerase (joint project with Ravindra Kumar & Suresh Kumar) CSIR, 2001-2005.

  12. RNA-protein Interaction in formation of measles Virus Nucleocapsid, UDC 2001-2004.

  13. Construction and Expression of ribozyme targeting RNA component of telomerase, CSIR, 2001-2005.  

  14. Rescue of recombinant measles virus with hetrologous peptides, DST, 2002-2005.

Awards and Honour

Membership

  1. Research Degree Committee, Life Sciences, CSJM University, Kanpur.

  2. School Board, Life Sciences, NEHU.

  3. Board of Studies in Botany, BHU.

  4. Selection Committee for Professor in Biochemistry, NEHU.

  5. Selection Committee for Professor in Molecular Biology, BHU.

  6. Chairman for multiple terms of Committees for Admission, Central Instruments Facility, Graduate Advisory Committee and Space etc. in the School of Life Sciences, JNU over 1990-current.

  7. Member of the Academic Council, JNU.

  8. Member of JNU Court.

  9. Chairman/member of Enquiry Committees constituted by the University with specific reference.

  10. Member of Selection Committees for professorial position in Central and State Universities.

Selected Publications

  1. Kumar, R., Dammai, V., Yadava, P.K., and Kleinau. S. (2005) .Gene targeting by ribozyme against TNF-a mRNA inhibits autoimmune arthritis. Gene Therapy. Jul 7 Epub ahead of print.

  2. Yadava, R.S., Kumar, R., and Yadava, P.K., (2005).  Expression of lexA targeted ribozyme in Escherichia coli BL-21(DE3) cells Molecular and Cellular Biochemistry, 271: 197-203.

  3. Preet, A., Gupta, B.L., Yadava, P.K., and Baquer, N.Z., (2005). Efficacy of lower doses of vanadate in restoring altered glucose metabolism and antioxidant status in diabetic rat lenses. Journal of Biosciences, 30: 221-230. 

  4. Chauhan, N., Kumar, R., Badhai, J., Preet, A., and Yadava, P.K., (2005). Immunogenicity of cholera toxin B epitope inserted in Salmonella flagellin expressed on bacteria and administered as DNA vaccine.  Molecular and Cellular Biochemistry. (In Press).

  5. Preet, A., Gupta, B.L., Siddiqui, M.R., Yadava, P.K., and Baquer, N.Z. (2005) Restoration of ultrastructural & biochemical changes in alloxan-induced diabetic rat sciatic nerve on treatment with Na3VO4 and Trigonella--- a promising antidiabetic agent. Mol Cell Biochem. (In Press).

  6. Dasgupta T, S. Banerjee, Yadava PK and Rao AR (2004) Chemopreventive potential of Azadirachta indica (neem) keaf extract in murine carcinogenesis model systems. J. Ethnopharmacol. 92:23-36.

  7. Dasgupta, T., Rao, A.R., and Yadava, P.K., (2004). Chemomodulatory efficacy of Basil leaf (Ocimum bacilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine, 11: 139-51.

  8. Dasgupta, T., Banerjee, S., Yadava, P.K., and Rao, A.R., (2004) .Chemopreventive potential of Azadirachta indica (neem) keaf extract in murine carcinogenesis model systems. J. Ethnopharmacol. 92:23-36.

  9. Dasgupta, T., Rao, A.R., and Yadava, P.K., (2003). Modulatory effect of Henna leaf (Lawsonia inermis) on drug metabollising phase I and phase II enzymes, antioxidant enzymes, lipid peroxidation and chemically induced skin and forestomach papillomagenesis in mice. Molecular and Cellular Biochemistry, 245: 11-22.

  10. Kumar, R., Yadava, P.K., and Kleinau, S., (2003). TNF-alpha targeting ribozyme as potential tool for gene therapy of rheumatoid arthritis. Scandinavian J. Immunol, 58: 229-230.

  11. Baquer NZ, Gupta D and Yadava PK (2003) Reversal of diabetic complications by vanadium and Plant Products. Proc. Symp. Trends Clin. Biochem. Lab. Medicine, 640-648.

  12. Yadava PK (2003) Emergence of nucleic acids as targets and tools for prophylaxis and therapy. XXVII Congress of Indian Academy of Social Sciences, IIT Kharagpur, Dec 3-7.

  13. Kumar R, Yadava PK and Kleinau S (2003) Construction and expression of a ribozyme targeting TNF-a results in TNF-a suppression in LPS-stimulated murine macrophage cell line. 5th Cambridge Symp on Nucleic Acid Chemistry and Biology, Queen’s College, Cambridge, UK, Aug 31-Sept 3.

  14. Dasgupta T,  Rao AR and Yadava PK (2003) Chemomodulatory efficacy of Basil leaf (Ocimum bacilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine 11:139-151.

  15. Anju Preet, Najma Zaheer Baquer and Pramod Kumar Yadava (2003) Regulation of glucose flux through polyol pathway and glycolysis in diabetic rat tissues: Effect of vanadate and Trigonella foenum graecum. 29th Annual Conference of Association of Clinical Biochemists of India, Feb 3-5, 2003, Jaipur.

  16. Anju Preet, Najma Zaheer Baquer and P. K. Yadava (2003) Modulation of the polyol pathway enzymes in diabetic rat tissues: Effect of vanadate and Trigonella foenum graecum. 2nd International Symposium on Diabetes, Jan 18-20, Mumbai.

  17. Ravindra Kumar, Mahima Arya, Suresh Kumar and Pramod Yadava (2002) New tools for studying cross talk between pro- and anti-apoptotic molecules. International Conference on Trends in Cancer Research, March 14-16, New Delhi.

  18. Yadava R., Vincent Dammai, Ravindra Kumar & Pramod Yadava (2003) Application of ribozymes in therapy and developing disease model. In: Trends in Animal Biotechnology (Srivastava AK ed.), Oxford University Press.

  19. Dasgupta T, Banerjee S, Yadava PK and Rao AR (2001) Chemomodulation of carcinaogen metabolising enzymes, antioxidant profiles and skin and forestomach papilomagenesis by Spirulina platensis. Molecular and Cellular Biochemistry 226, 27-38.

  20. Raju J, Gupta D, Rao AR, Yadava PK and Baquer NZ (2001) Trigonella foenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic and lipogenic enzymes. Molecular and Cellular Biochemistry 224: 45-51.

  21. Baquer NZ, Gupta D and Yadava PK (2001) Reversal of diabetic complications by vanadium and plant products. ( th Asian Pacific Congress of Clinical Biochemistry, 11-16 November 2001, New Delhi.

  22. Bhattacharya P and Yadava PK (2000) Subunit peptide and genetic vaccination with measles virus. Molecular Biology Today 1:80-95.

  23. Suresh Kumar R and Yadava PK (2000) Telomeres, cell senescence, aging and cancer . National Symposium on Cellular and Molecular Biiology of Aging, School of Life Sciences, JNU, New Delhi, Oct 20-21.

  24. Chander A, Yadava R, Ghosh S, Rath PC, Yadava PK, Orme I and Saxena RK (2000) Strain differentiation among Indian clinical isolates of M. tuberculosis by RFLP analysis. Molecular Biology Today, 1, 53-55.

  25. Yadava R and Yadava PK (2000): Vectors for generation of precise transcripts of inserted sequence. Molecular Biology Today, 1, 17-22.

  26. Yadava, PK (2000) Nucleic acid therapeutics: Current targets for antisense oligonucleotides and ribozymes. Molecular Biology Today, 1, 1-16.

  27. Baquer NZ, Gupta D and Yadava PK (2000) Reversal of diabetic complications by vandium and plant derived antidiabetic compounds. 18th International Congress of Biochemistry and Molecular Biology, Birmingham, UK.

  28. Raja V, Yadava PK and Sarin NB (2000) Cloning meristem-specific cDNA from Cajanus cajan. International Congress of Plant Molecular Biology, Ottawa.

  29. Ravindra Kumar, Vincent Dammai and Pramod K Yadava, (1998) Cloning and purification of histidine-tagged human TNF-alpha from Escherichia coli. 67th Annual Meeting of the SBC, Dec 19-21, JNU, New Delhi.

  30. Vincent Dammai1 Prem N.S. Yadava2 and Pramod K. Yadava, (1998) Dimerization of a hammerhead ribozyme by certain proteins: Looking for common RNA-interactive motifs.  67th Annual Meeting of the SBC, Dec 19-21, JNU, New Delhi.

  31. Pramod K Yadava (1998) Signs of self-templated growth in RNA virus genomic sequences. 67th Annual Meeting of the SBC, Dec 19-21, JNU, New Delhi.

  32. Pramod K Yadava (1998) Development of therapeutic molecules based on the measles virus reverse genetic system. 67th Annual Meeting of the SBC, Dec 19-21, JNU, New Delhi.

  33. Yadava PK and Dammai VRK (1998) Expression of hammerhead ribozyme diminishes apoptotic factor in culture supernatant of a TNF-a producing cell line THP1. Antisense 98, Oct 8-9, 1998, London.

  34. Dammai V and Yadava PK (1998) Modulation of TNF-a  gene function with the help of targeted ribozyme. X International Congress of Immunology, Nov 1-6, New Delhi, INDIA.

  35. Yadava PK (1998) Drifting towards technology: Compulsions and options. Keynote address delivered at National Symposium on 50 years of Botany in India: An assessment, Centre for Advanced Studies in Botany, BHU, February 27-28, 1998.

  36. Yadava RS, Chander A, Siddiqui Z, Rath PC, Yadava PK & Saxena RK (1997) Novel repeat DNA sequences cloned from an Indian strain of M. tuberculosis. International Symposium on Trends in Life Sciences, JNU, New Delhi.

  37. Yadava RS, Rajkumar VD & Yadava PK (1997) Construction and characterization of biomolecules in prophylaxis and therapy: I. Targeted inactivation of gene function using cognate ribozymes. International Symposium on Trends in Life Sciences, JNU, New Delhi.

  38. Yadava, RS, Chander, A, Rath, PC, Yadava, PK & Saxena, RK (1997) Novel repeat DNA sequences cloned from an indian strain of M. tuberculosis.  National Immunology Conference, Bose Institute at Calcutta from Dec. 22-25, 1997.

  39. Chauhan N, Srivastava BS & Yadava PK (1997) Construction and characterization of biomolecules in prophylaxis and therapy: II.Chimeric flagellin as promising vaccine carrier. International Symposium on Trends in Life Sciences, JNU, New Delhi.

  40. Yadava PK & Billeter MA (1997) Construction and characterization of biomolecules in prophylaxis and therapy: III. Measles virus reverse genetic system. International Symposium on Trends in Life Sciences, JNU, New Delhi.

  41. Yadava PK (1997) Construction and characterization of biomolecules in prophylaxis and therapy: IV. Measles virus pseudotypes as targeted delivery system.  International Symposium on Trends in Life Sciences, JNU, New Delhi.

  42. Chauhan N & Yadava PK (1997) chimeric flagellin with insert coding for an epitope of cholera toxin B. Indo-French Symposium on Immunopharmacology, National Institute of Immunology, New Delhi.

  43. Yadava RS & Yadava PK (1997) Targeted ribozymes as model tools for trans regulation of gene expression: Studies with lexA of Escherichia coli. in Biochemistry, Biophysics and Molecular Biology Section Indian Science Congress, New Delhi.

  44. Yadava RS, Rajkumar VD & Yadava PK (1997) Expression of a hammerhead ribozyme targeted against lexA in Escherichia coli results in derepression of recA. 17th International Congress of Biochemistry and Molecular Biology, San Francisco (Aug 24-29, 1997).

Administration and related experience:

  1. Warden. Narmada Hostel, JNU, 1991-1995.

  2. Provost, Pashchimabad (3 Hostels), JNU, 1993-94.

  3. Warden, Kavery Hostel, JNU, 1995-2002.

  4. Provost, Pashchimabad (5 Hostels), JNU, 2001-2004.

Others

  1. Invited to deliver keynote addresses in symposia organized in Banaras Hindu University, University of Allahabad, Indian Veterinary Research Institute, Izatnagar.

  2. Invited for lectures for M.Sc. Biotechnology, Life Sciences and Microbiology at the following Universities: (a) Banaras Hindu Univrsity, (b) Delhi University, (c) North Eastern Hill University, (d) Jiwaji University, Gwalior, (e) Barkatullah University, Bhopal, (f) M.S. Univresity, Vadodara, (g) Gorakhpur University, (h) Bhagalpur University, (i) Utkal University, Bhubaneswar,  (j) Gorakhpur University etc.

  3. Served as member of Selection Committees for Professorial positions in Central and State Unviersities.

  4. Served as Examiner for PG courses in Biochemistry, Life Sciences and Biotechnology in Jiwaji University, Barkatullah University, Guru Nank Dev University, Jamia Hamdard University, Banaras Hindu University, Gorakhpur University, CSJM University of Kanpur, North Eastern Hill University.

  5. Served as Examiner of doctoral theses from BHU, CSJMU, Lucknow University, Jadavpur University, Bhavnagar University etc.

  6. Served as Member of Board of Studies/Research Degree Committees at BHU, NEHU, CSJMU, Jamia Hamdard University.

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