Prof. K.J. Mukherjee
 

Dr. K. J. Mukherjee
Professor and Dean
Ph: +91 11 26704087
Email: kjmukherjee@mail.jnu.ac.in

Education

Ph.D. (Biochemical Engg.), IIT, New  Delhi.
M.Tech. (Biochemical Engg.), IIT, New Delhi.
B.Tech. (Chemical Engg.), IIT, Kanpur

Career:

2008-
Professor, School of Biotechnology, JNU, New Delhi.
2000-2008
Associate Professor, Special Centre for Biotechnology, JNU, New Delhi.
1991-2000
Assistant Professor, Special Centre for Biotechnology, JNU, New Delhi.

Area of Interest:

  • Metabolic engineering for redesign of hosts as an ideal platform for recombinant protein expression
  • Bioprocess monitoring and modeling of recombinant cultures
  • Scale-up of recombinant protein production

Summary of Research:

Our work is at the interface of molecular biology and biochemical engineering, where genetic engineering tools are used to construct a range of host-vector combinations required to over-express recombinant proteins and these are then tested and optimized in a bioreactor. We have also initiated work in metabolic engineering where the host for expression is redesigned so as to divert the metabolic fluxes towards recombinant protein synthesis. We also do online process monitoring utilizing biophysical tools like fluorescence, capacitance, NIR, etc. to study and model the metabolic state of the cell. Since we work with therapeutically important recombinant proteins these find natural use in pharmaceutical industry. We have initiated many University-Industry collaborations where long-term projects have been set up leading to technology transfer

Honors/Awards:

Commonwealth fellowship for  at the Deptt. of Genetics, Univ. of Cambridge, Cambridge, UK (Oct. 01,  2001 – Sept.  30, 2002).

Selected Publications:

  1. R. Walia, J. K. Deb, and K. J. Mukherjee (2008). Stability studies with different vector backbones utilizing the T7 expression system in Escherichia coli. J. Chem.  Technol.  Biotech.,   83:1120-1125
  2. Y. P. Khasa, A. Khushoo, L. Srivastava and  K. J. Mukherjee (2007). Kinetic studies of constitutive hGM-CSF expression in continuous culture of Pichia PastorisBiotechnol. letters  29: 1903-1908.
  3. R. Walia, J. K. Deb and K. J. Mukherjee (2007). Development of expression vectors for Escherichia coli based on the pCR2 replicon. Microbial Cell Factories, 6:14.
  4. P. Bhattacharya, G. Pandey, P. Srivastava and  K. J Mukherjee (2007). Production and purification of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) from high cell density cultures of Pichia pastoris.  Bioprocess and Biosystem Engg. 30: 305-312.
  5. Y. Pal, A. Khushoo and  K. J. Mukherjee (2006). Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture. Appl. Microbiol. Biotechnol., 69: 650-657.
  6. P. Srivastava and K. J. Mukherjee (2005). Kinetic studies of recombinant human Interferon-alpha (rhIFN-alpha) expression in transient state continuous cultures.  Biochemical Engg.  J.,   26:  50-58.
  7. A. Khushoo, Y. Pal and K. J. Mukherjee (2005). Optimization of extracellular production of recombinant asparaginaase in Escherichia coli in shake-flask and bioreactor. Appl. Microbiol. Biotechnol., 68:189-197.
  8. P. Bhattacharaya, G. Pandey, P.  Srivastava and  K. J. Mukherjee (2005). Combined effect of protein fusion and signal sequence greatly enhances the production of recombinant GMCSF in E.coli. Mol. Biotechnol.,  30: 103-116
  9. P. Srivastava, P. Bhattacharaya, G. Pandey and  K. J. Mukherjee (2005).  Overexpression and purification of recombinant human Interferon alpha2b in Escherichia coli. Protein Expr. Purif.,  41:313-322.
  10. A. Khushoo, Y. Pal and K. J. Mukherjee (2005). Extracellular expression and single step purification of recombinant Escherichia coli L-asparaginase II. Protein Expr. Purif.,    38: 29-36.
  11. K. J. Mukherjee, D. C. D. Rowe, N. A. Watkins and D. K. Summers (2004). Studies on Single Chain Antibody Expression in Quiescent Escherichia coli. Appl. Env. Microbiol.,  70: 3005-3012

 

 

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